Translational_Unit

Part:BBa_K4796009:Design

Designed by: Brenda Ma   Group: iGEM23_UBC-Vancouver   (2023-09-26)


Nrdj-1 Split intein N terminal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 68
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensure that Nrdj-1 split intein N and C terminal are encoded on the same plasmid flanking a CDS for peptide expression to allow post translational splicing and rejoining to take place.


Source

Derived from Mycobacterium xenopi

References